This study evaluates a number of techniques that influence the accuracy and precision of the pipetted volume. The pipette operator has the ability to control all of these parameters by using the appropriate pipetting technique, as well as by choosing the appropriate pipette size and type of pipette tips.
Every day, air-displacement pipets are used to quantitatively dispense sample and reagent aliquots for reaction, routine analyses, and specialized tests. Since concentrations of biological and chemical components in the prepared samples for analyses and assays are volume-dependent, incorrectly performed pipetting steps will directly impact the transferred volumes, and hence, the test results.
Working with Artel, Takeda developed an analyst qualification program using the same technology it uses to verify and calibrate its robotic liquid handlers. The implementation of the comprehensive program not only reduced assay failures but also helped the company transfer assays from bench to automation by using the same measurement standard for each phase of the process.