In this webinar, Dr. Nathaniel Hentz and Jason Graves will highlight their approach for addressing NGS workflows and present methodologies for improving DNA normalization, ensuring accuracy of master mix addition, studying mixing efficiency and improving operator technique.
By understanding the key steps in this assay workflow, variability can be minimized upfront allowing an easier method transfer to an automation platform. This tutorial demonstrates the importance of understanding the assay technicalities that should be taken into consideration before automated scripts are created to prepare for potential challenges encountered during method transfer.
Scientists typically optimize assay parameters first, then select an automated liquid handler using default parameters during transition to an automated platform. This study illustrates the importance of optimizing both the assay and any associated liquid handling steps with the goal of minimizing variability.