Mixing of solutions in 384-well microplates is a challenging, yet highly important task for many laboratories and assays. Effective mixing is of particular importance for volume verification using the MVS where two dyes must be completely mixed before photometric measurements are made.
You wouldn’t bake a cake without mixing the ingredients first, yet when important assays are being conducted the liquids dispensed into a microplate are often assumed to have mixed through simple diffusion or after only a cursory agitation of the plate.
Efficient mixing of solution components is a critical step in the performance of certain assay types. As more life science and pharmaceutical laboratories conduct their assays in 96- and 384-well plate formats, mixing issues within these plates are becoming more common than ever before