This study uses a two factor design (temperature and humidity) to investigate volumetric performance (precision and trueness) at environmental conditions from 15 °C to 30 °C and 30% to 80% relative humidity. Nine environmental conditions were tested, the mid point, four corner cases and four edge cases. Replicate testing was performed at the mid point and corner cases.
Understanding how pipettes perform in high humidity is critical for laboratory data integrity. According to regulatory standards, pipette calibration laboratories must control their relative humidity at greater than 50%, while working laboratories are often significantly drier.