This study uses a two factor design (temperature and humidity) to investigate volumetric performance (precision and trueness) at environmental conditions from 15 °C to 30 °C and 30% to 80% relative humidity. Nine environmental conditions were tested, the mid point, four corner cases and four edge cases. Replicate testing was performed at the mid point and corner cases.
Understanding how pipettes perform in high humidity is critical for laboratory data integrity. According to regulatory standards, pipette calibration laboratories must control their relative humidity at greater than 50%, while working laboratories are often significantly drier.
Many common laboratory procedures require the handling and quantitative dispensing of reagents at various temperatures. Mechanical action micropipettes are most often used for this routine task. The construction of these pipettes, however, makes their performance susceptible to variations in temperatures of the samples dispensed.
Many common laboratory procedures require quantitative pipettes and mechanical action air-displacement micropipettes are frequently used for this purpose. The design of these pipettes, however, makes their performance susceptible to sample temperature, as well as to environmental relative humidity and barometric pressure.
In light of the multitude of error sources, it is easy to overlook the environmental conditions in the laboratory, and the effects they can exert on the quantitative dispensing of liquid with handheld pipettes.