Assay-ready plates are becoming key to maximizing throughput and assay robustness for many HTS departments. These plates contain test compounds prepared at specific concentrations from library stocks ahead of time.
Takeda California uses the non-contact ATS-100 Acoustic Transfer System in various compound management liquid handling activities in support of drug discovery processes. It is widely accepted that proper quality assurance is important for accurate and efficient processes - and proper calibration of automated liquid handling instruments is a key component of such quality assurance.
This poster examines the differences in liquid delivery by automated liquid handlers dispensing both aqueous and DMSO-based test solutions. For the study discussed herein, an ALH was first optimized to dispense aqueous test solutions, after which these optimized aqueous method(s) were used to dispense identical target volumes of both DMSO- and aqueous-based MVS Sample Solutions into microtiter plates, which were then measured with the MVS to determine actual delivered volume.
Acoustic transfer systems operate by ejecting a rapid fire stream of tiny discrete droplets, with each droplet adding a few nanoliters to the total aliquot. This makes the system digital, in the sense that volumes can be changed by adjusting the number of droplets.