September 20, 2013

Minimizing Liquid Delivery Risk: Laboratory Environmental Conditions as Sources of Error

In light of the multitude of error sources, it is easy to overlook the environmental conditions in the laboratory, and the effects they can exert on the quantitative dispensing of liquid with handheld pipettes.
September 20, 2013

In Liquid Handling Quality Assurance, All That Matters is Location, Location, Location

Regulations are not keeping pace with new technology, leading to a lack of relevant calibration standards or guidelines. Laboratories need to update their calibration procedures to measure and document microliter/nanoliter volumes quickly and accurately.
September 20, 2013

The Use of A Dual Dye Photometric Calibration Method To Identify Possible Sample Dilution From An Automated Multichannel Liquid-Handling System

A series of experiments, using the default pipetting parameters, was performed which demonstrated that the difference in the reported volume was due to a dilution effect by the system liquid of the liquid handler and did not indicate an error in the delivered volumes.
September 20, 2013

Verifying Liquid Handler Performance for Complex or Non-Aqueous Reagents: A New Approach

Accurate and reliable adjustment of liquid handler protocols for varied reagent types could have far reaching adoption in all scientific communities. Using this methodology, target aliquots of multiple solutions were dispensed with a calibrated syringe and subsequently validated via dual-dye ratiometric photometry.
September 19, 2013

Importance of Integrating a Volume Verification Method for Liquid Handlers: Applications in Learning Performance Behavior

Nearly all assays performed within a microtiter plate are volume dependent. In turn, all concentrations of biological and chemical components in these assays, as well as […]
September 19, 2013

Determining Dilution Accuracy in Microtiter Plate Assays Using Quantitative Dual-Wavelength Absorbance Method

This paper describes a new approach, which is based on dual dye, dual-wavelength photometry, for accurately measuring each dilution step in a multistep dilution protocol. The theory behind this method is described, as well as an experimental demonstration of the prescribed approach.
September 7, 2013

Metrological Traceability of In-House Calibrations

Equipment calibration is a necessary and common fact of life in regulated laboratories, and the decision on how to calibrate often begins with a simple choice […]