In order to obtain promising HTS candidates, high quality assays are required. Assay variability is a combination of liquid handling, biological and random variability. Liquid handling variability is often underappreciated, yet can have a huge impact on the outcome of an assay.
Automation has certainly led to increased numbers of experiments, as compared to manual testing, particularly for pipetting operations. Because of this advantage, liquid handling robots have become commonplace even in small laboratories.
The volume verification method, or volume transfer evaluation process, should be standardized, fast, easy to implement, and should minimize instrument downtime and required resources (labor, reagents). Additionally, volume transfer for critical target reagent screening should be compared for all devices within a process.
Users of automated liquid handlers need to ensure the accuracy and precision performance of their equipment in order to satisfy their quality system and regulatory requirements. However, for most users, equipment calibration and performance verification activities are time-consuming, tedious and not well standardized.
All users of automated liquid handling equipment are familiar with the need to ensure proper performance of their equipment. But for most users, calibration and performance testing is time consuming and burdensome.
Caliper Life Sciences Liquid Handling Equipment allows for easy optimization of aspirate/dispense protocols , ensuring precise and accurate instrument performance. This demonstration will highlight not only the ease of use of the MVS, but also the performance capabilities of Caliper equipment, and the benefit of validating critical test protocols.
Accuracy Matters When Transferring a Quantitative, Bench-top Assay to Automation Introduction Assays are usually performed on the benchtop using handheld pipettes before they graduate, or transfer, to […]
Many critical assays are based upon conducting accurate dilutions of key reagents. Accurately knowing sample concentration is critical for properly interpreting experimental results, which can only be obtained if the experimental dilution ratio is known and controlled.