September 28, 2013

Accuracy of Serial Dilutions Tested Over a Large Dilution Range

The process of serially diluting solutions in microplates is common for many assays including preparation of dose response curves for IC50, drug efficacy testing, etc. There are many critical steps to this type of assay that are carefully controlled, such as contaminant quantitation and prospective drug concentration.
September 27, 2013

Rapidly Quantifying Dilutions in Microtiter Plates

Dilution-based volume transfer steps are commonly performed for many applications, such as dose response and detection limit testing. Because the exact concentrations of target samples in solution are typically not directly measured but assumed from the dilution ratio used, it is important to accurately measure each dilution step so that the ultimate concentrations of those samples are known.
September 26, 2013

Simple and Fast Performance Verification for Ultra-Low-Volume Liquid Handlers

Poor liquid handling performance can lead to poor assay results. When the volume verification methodology used to assess liquid handler performance is not properly executed, errors will enter into the process and the results could lead to incorrect conclusions regarding instrument performance.
September 25, 2013

The Effects of Pipette Tip Production & Sterilization Methods on the Retention of Liquids

Plastic pipette tips are ubiquitous pieces of labware commonly used in life science, pharmaceutical, chemistry and many other types of laboratories. Because of their use in such diverse fields, certain qualities have been implemented to meet specific needs. Some of these qualities include sterilization of the tips for biological work, the addition of filters inside the tip to reduce aerosol contamination of the pipette cavity, and reducing the volume of drops retained by the tip’s surface.
September 23, 2013

Errors in Liquid Delivery

Many common laboratory procedures require quantitative pipettes and mechanical action air-displacement micropipettes are frequently used for this purpose. The design of these pipettes, however, makes their performance susceptible to sample temperature, as well as to environmental relative humidity and barometric pressure.
September 22, 2013

Uncovering Dimensional Variability in Standard Microtiter Plate Types

First created in the 1950’s, microplates have become so common place that in 2004, the Society for Biomolecular Screening (SBS) established standards defining multiple dimensions within a plate.
September 20, 2013

Painless Nanoliter-Transfer Accuracy Inspection for Busy Screeners

Acoustic droplet ejection first provided low-nanoliter transfers for high-throughput screening applications in 2004. At that time, there were no methods that could quickly and accurately determine the accuracy and precision of the transfer volume.
September 7, 2013

Photometric Measurement of Mixing Efficiency Using The Eppendorf MixMate Plate Mixer

Efficient mixing of solution components is a critical step in the performance of certain assay types. As more life science and pharmaceutical laboratories conduct their assays in 96- and 384-well plate formats, mixing issues within these plates are becoming more common than ever before