September 30, 2013

Operators as Sources of Error

Many regulatory bodies have established guidelines designed to help laboratories achieve and maintain good quality practices, while providing a sense of confidence in the quality of work performed at an accredited facility.
September 30, 2013

Optimize Volume Transfer Methods to Avoid Reagent Concentration Error

Laboratories utilizing liquid handling devices appreciate the efficiency benefits that these important instruments provide. Fast and repeatable volume transfers, however, do not always contribute to accurate assay results. Assays are dependent on reagent concentrations and reagent concentrations are volume- dependent.
September 30, 2013

Errors Associated with Pipetting of Warm and Cold Liquids

Many common laboratory procedures require the handling and quantitative dispensing of reagents at various temperatures. Mechanical action micropipettes are most often used for this routine task. The construction of these pipettes, however, makes their performance susceptible to variations in temperatures of the samples dispensed.
September 30, 2013

Best Practices for Assessing Nanoliter Transfer Performance for Labcyte Echo Liquid Handlers

Acoustic drop ejection (ADE) technology enables low- nanoliter transfers for high-throughput screening applications. Verifying the absolute transferred volume and the well-to-well reproducibility of the ADE liquid handler is important for quality control, but there are few methods available that can quickly and accurately interrogate dispensed volumes in this range.
September 30, 2013
Rapid Accuracy

Rapid Accuracy and Precision Assessment of DMSO Compound Solutions Transferred with Pocket Tips

Performing assays with liquid handling equipment to rapidly test and screen compounds is essential to drug discovery and other biopharma processes. In order to increase the productivity and integrity of such screenings, it is important to understand how accurately and precisely target volumes are being transferred [...]
September 30, 2013

Verifying Mosquito Nanoliter Liquid Dispensing

Assay-ready plates are becoming key to maximizing throughput and assay robustness for many HTS departments. These plates contain test compounds prepared at specific concentrations from library stocks ahead of time. DMSO is routinely used for compound dissolution, but its presence at concentrations above 1% can markedly affect assay performance.
September 30, 2013

Development of a 100% DMSO Sample Solution for Liquid Handler Performance Verification

DMSO (dimethyl sulfoxide) has become one of the most important solvents used for high-throughput screening (HTS) and compound management applications due to its ability to readily dissolve a wide range of compounds. It is also the solvent of choice for the new breed of acoustic droplet ejection (ADE) liquid handlers, developed by companies such as Labcyte and EDC Biosystems, which have revolutionized HTS and compound management.
September 28, 2013

Automation of Nanoliter Volume Transfers

Doing More With Less Introduction To facilitate the discovery and refinement of lead compounds, our laboratory known as the Facility for Automation and Screening Technology (FAST) […]
September 28, 2013

Quantifying the Impact of Operator Error in Pipetting

Mechanical action hand-held pipettes are commonly used for transfer of microliter liquid quantities in a variety of analytical laboratories including pharmaceutical quality control, medical testing, forensic analysis, and food safety laboratories. Accurate and precise analytical results from these laboratories are necessary to protect consumers, instill confidence, and support global trade.
September 28, 2013

Accuracy Matters When Quantitative, Manually-pipetted Assays Graduate to Automation

A Story in Diagnosing and Troubleshooting Abstract It is often the case that assays are initially performed on the benchtop  using handheld  pipettes  before they graduate, […]