The focus of this application note is on volume verification and subsequent optimization; it does not speak to the quality of volume transfer performance you should expect from any Preddator. Pipetting accuracy can be improved in multiple ways on the Preddator, such as by adjusting tip-by-tip calibration factors, system pressure settings, varying solenoid size, etc.
A common interest in all areas of life science: lower costs and reduce repeat testing. Removing sources of variability from test processes reduces error leading to rework, lowers risk and increases reliability of the results. One likely source of variability, which is easily controlled, is liquid handling.
This application note discusses one approach for optimizing pipetting accuracy on a Beckman Coulter Biomek liquid handler using MVS measurement information. Pipetting accuracy can be improved in any number of ways, such as by adjusting individual liquid class variables (aspirate rates, dispense rates, air gaps, mix steps, etc.) and measuring transferred volume resulting after each successive adjustment.
This application note discusses one approach for optimizing pipetting accuracy on an Agilent Bravo liquid handler. Although the MVS system supports verifying both tip-by-tip precision and accuracy values for nearly all liquid handlers, the focus of this paper is accuracy measurement and optimization.
With budgets forever tightening and patient samples being limited, increased efficiency and reduction of repeat testing has become a common goal of almost all laboratories regardless of their operation. This has led to laboratories introducing automated liquid handling systems to reduce the variability introduced by human operators during manual pipetting steps.
Custom test liquids using assay-specific reagents can be prepared and employed to assess volume transfer performance of a liquid handler using the Artel MVS® Multichannel Verification System. This application note describes general methods for creating and testing custom test solutions, referred to as alternative test solutions.
An integral, yet often overlooked, procedure with any given assay, washing steps are vital to ensure reaction quality and integrity particularly when conducted in a microtiter plate. Whether it is complete removal of unbound material within an ELISA, effective evacuation of residual wash buffer solution, or even simple removal of supernatant such as cell media just above the surface of adherent cells, effective washes can make a significant difference in the final results.
All pipette tips are not created equal, nor are all assays. Failure to use proper tips for a given type of pipette can lead to an inadequate seal between the pipette and tip, causing inaccurate volume transfers due to leakage and sample loss. The need for the best performing tip is likely proportional to the importance of having both accuracy and precision in volume delivery for specific assay steps.
The purpose of this application note is to demonstrate an alternative use for the MVS. As opposed to measuring the accuracy and precision for a volume transfer of sample into a microtiter plate, the MVS is instead employed to measure the volume of sample left behind after attempting to aspirate off the entire sample from each well of a microplate, i.e., residual volume of sample.