This application note demonstrates an approach for rapidly optimizing a liquid class to dispense PlasProxy&trade – a new MVS® QualAssure® solution that mimics the pipetting characteristics of various types of plasma – using an automated liquid handler (ALH). Utilizing the Multichannel Verification System (MVS) is crucial for conducting liquid class optimization quickly and efficiently.
The purpose of this application note is to demonstrate an alternative use for the Artel MVS® Multichannel Verification System. As opposed to measuring the accuracy and precision for a volume transfer of sample into a microtiter plate, the MVS is instead employed to measure the volume of sample left behind after attempting to aspirate off the entire sample from each well of a microplate, i.e., residual volume of sample.
As more and more researchers move to sub-10 μL reaction volumes in their genomics workflows, it will become more critical than ever before to ensure highly precise liquid handling within these volume ranges. The MANTIS can be easily added to any traditional liquid handling workstation in order to obtain CV’s under 2% across the entire pipetting range.
This application note provides performance testing results obtained at the low volume range capability of Beckman Coulter’s Biomek i-Series Automated Workstation. The Artel MVS was used to measure the delivered volumes and to facilitate the optimization of pipetting techniques through Biomek Software.
Today’s biotech, pharmaceutical, environmental, clinical research, and agricultural laboratories employ a diversity of applications and depend more and more on automated systems to address the dynamic pipetting needs for these assays. Regardless of the application, users demand a high degree of accuracy and precision of the pipetting platform to ensure confidence in the final result.
A precise and accurate means for conducting volume verification of automated liquid handlers is an important requirement in life science laboratories, which need to determine if pipetting performance is within expected parameters and meets the manufacturers' published specifications.
Ensuring that pipetting devices (both manual and automated) are performing properly is often a regulatory requirement. Many of those labs that are not required to prove such documentation will proactively encourage that a quality assurance program exists and is followed within their labs as pipetting errors can lead to costly amounts of rework and damage reputations.