Understanding Method Transfer Pitfalls

MVS Liquid Class

Accuracy Matters When Transferring a Quantitative, Bench-top Assay to Automation


Assays are usually performed on the benchtop using handheld pipettes before they graduate, or transfer, to an automated liquid handler. Automating a manual method may take time and patience, but automation helps lower costs, increase throughput, and potentially avoids errors associated with a manual method. During the transfer, however, the manual assay should be directly compared to the automated assay for consistencies in pipetting performance. Beckman Coulter and Artel jointly discuss the importance of diagnosing liquid handling variability during method transfer because an undetected difference in accuracy will impact the integrity of the assay as the automation process continues.

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