Acoustic drop ejection (ADE) technology enables low- nanoliter transfers for high-throughput screening applications. Verifying the absolute transferred volume and the well-to-well reproducibility of the ADE liquid handler is important for quality control, but there are few methods available that can quickly and accurately interrogate dispensed volumes in this range. When the volume verification method used to assess transfer performance is not analytically implemented and properly executed, or when it is rushed through due to time constraints, unseen errors may occur in the results which may introduce a false sense of transfer performance.
This poster presentation discusses the best practices for assessing liquid transfer accuracy and precision for the Echo® 555 liquid handler (Labcyte Inc.) with the Artel MVS Multichannel Verification System, a standardized volume verification platform using a dual-dye absorbance- based method. Some of the best practice topics discussed herein include test solution preparation, source plate preparation and assay plate reagent mixing. By following these recommended practices, optimal conditions for measuring the Echo performance can be achieved. Using the best practices, the measured accuracy and precision of the Echo were below 5% inaccuracy and 5% CV for the transfer volumes of 50 to 300 nL of DMSO solutions.View Poster