Automation scientists routinely work with complex liquids from development through production. Optimizing an automated liquid handler to dispense one fluid type is difficult enough, but for systems that dispense multiple liquids with vastly different properties the challenge is even greater. To help with this, automated liquid handler manufacturers provide default liquid classes to dispense different fluid types.
However, most automation engineers know that the default liquid classes serve as a good starting point and those liquid classes must often be optimized relative to the working environment. Another challenge facing automation engineers is how to measure the liquid dispense during optimization. We will discuss the benefits of combining our easy four-step process with our dual-dye photometric method to quickly optimize a liquid class in an automated liquid handler. Liquids as diverse as DMSO, PCR master mix, serum, and plasma will be discussed.