A New Solution for Optimizing PCR Master Mix Liquid Transfers

A-New-Solution-for-Optimizing-PCR-Master-Mix-Liquid-Transfers

A-New-Solution-for-Optimizing-PCR-Master-Mix-Liquid-TransfersPrimary Author: Michael Grimaldi, (Artel)
Co-Authors: Rachel Parshley, Heather Bushie, Kaziya Lee, PhD., John Thomas Bradshaw, PhD. (Artel)

Measuring and ensuring accuracy and precision of liquid delivery steps when transitioning from handheld pipettes to automated liquid handlers is a constant challenge, especially for complex assays like qPCR and next generation sequencing. In this poster we show the similarities of PCRMix QualAssure compared to various commercially available PCR and qPCR master mixes. Equally, we show that aqueous and glycerol/water solutions are not suitable mimics for PCR master mix.