Resource Library: Knowledge and practical liquid handling tips you can put into practice

The Digest brings you technical resources, expert tips, guidance on regulations and standards, as well as discussions with key industry leaders – that focus on optimized assay workflows, automated liquid handling and pipetting processes.

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Process Optimization for Assays - Whitepaper
Find out how by downloading this new White Paper on using process optimization to reduce assay variability and improve productivity.
Pipetting Viscous Solutions Poster
Pipetting Viscous Solutions
The Pipetting Viscous Solutions poster provides guidance on pipetting solutions and helps in understanding that viscosity is the measure of a liquid’s resistance to flow.
Troubleshooting Automation
Troubleshooting Automation
This Troubleshooting Automation poster provides guidance on how to identify common problems so you can get the best performance out of your liquid handling platforms.

Improving Assay Performance Using a Process Optimization Approach

By Hentz, N., Avis, E.
Abstract High throughput screening (HTS) campaigns often rely on a single test to make a decision to proceed onto the next step. Minimizing assay variability…

Does weather affect pipetting? YES!

By Rodrigues, G., Avis, E., Price, A., Gerber, V.
Note: This poster has been formatted to fit this screen. Click here to download the published version. Introduction Liquid handler performance can be optimized by adjusting…

Standardization of Tecan Freedom EVO liquid handling platforms using the QC Kit

By Albert, K., Rodrigues, G., Sullivan, T.
Description Variability across liquid handling platforms of the same make and model is often uncharacterized and can lead to erratic results from instrument to instrument…

Introducing New Technology for Liquid Handling Quality Assurance

By Gigante, B., Bradshaw, J.
Description Controlling and optimizing the liquid handling component of drug discovery and other life science laboratory processes can save valuable time and resources. This tutorial…

Effect of Liquid Handling Accuracy on Assay Performance

By Hentz, N., Knaide, T.
This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance…

Lab Report 1: How Many Data Points

By Artel
Human beings are creatures of habit We often seek stability and continuity and are wary of change. However, inspection, evaluation, and subsequent change are often…

Five Good Reasons: the argument for pipetting technique training

By Carle, Bjoern
Liquid handling quality assurance is a critical component of an effective laboratory quality program. While quality assurance efforts in liquid handling typically place much emphasis on pipette calibration, repair, and…

Optimize Volume Transfer Methods to Avoid Reagent Concentration Error

By Albert, K.
Note: This poster has been formatted to fit this screen. Click here to download the published version. Abstract Laboratories utilizing liquid handling devices appreciate the efficiency…

Accuracy Matters When Quantitative, Manually-pipetted Assays Graduate to Automation

By Albert, K., Knapp, L.
A Story in Diagnosing and Troubleshooting Abstract It is often the case that assays are initially performed on the benchtop  using handheld  pipettes  before they…

Accuracy of Serial Dilutions Tested Over a Large Dilution Range

By Knaide, T., Parshley, R., Bradshaw, J., Curtis, R.
ABSTRACT The process of serially diluting solutions in microplates is common for many assays including preparation of dose response curves for IC50, drug efficacy testing,…

Rapidly Quantifying Dilutions in Microtiter Plates

By Knaide, T., Bradshaw, J., Rogers, A., Albert, K.
Abstract Dilution-based volume transfer steps are commonly performed for many applications, such as dose response and detection limit testing. Because the exact concentrations of target…

Simple and Fast Performance Verification for Ultra-Low-Volume Liquid Handlers

By Albert, K., Lee, H.
Nanoliter Aqueous Fluid Transfers Assessed with Dual-Dye Technology Abstract Poor liquid handling performance can lead to poor assay results. When the volume verification methodology used to assess…

Understanding Method Transfer Pitfalls

By Knapp, L., Albert, K.
Accuracy Matters When Transferring a Quantitative, Bench-top Assay to Automation Introduction Assays are usually performed on the benchtop using handheld pipettes before they graduate, or transfer,…

Determining Dilution Accuracy in Microtiter Plate Assays

By Bradshaw, J.T.
Description Many critical assays are based upon conducting accurate dilutions of key reagents. Accurately knowing sample concentration is critical for properly interpreting experimental results, which can only be…

The Effects of Pipette Tip Production & Sterilization Methods on the Retention of Liquids

By Spaulding, B.W., Bradshaw, J.T., Albert, K., Campbell, D., Rodrigues, G.
Introduction Plastic pipette tips are ubiquitous pieces of labware commonly used in life science, pharmaceutical, chemistry and many other types of laboratories. Because of their…

Measuring Residual Volumes Remaining in a Plate After Sample Aspiration

By Khovananth, K., Neilsen, J., Albert, K.
Abstract The purpose of this application note is to demonstrate an alternative use for the Artel MVS® Multichannel Verification System. As opposed to measuring the…

Artel MVS as a Tool for Measuring Liquid Mixing Efficacy in Microplates

By Knaide, T., Parshley, R., Wilkinson, M.
Abstract You wouldn’t bake a cake without mixing the ingredients first, yet when important assays are being conducted the liquids dispensed into a microplate are…

Errors in Liquid Delivery

By Carle, B., Rumery, D., Davis, A., Sawyer, G., Albert, K., Bradshaw, J.
The Importance of Laboratory Conditions on Pipetting [one][one_second] Many common  laboratory procedures require quantitative pipettes and mechanical action air-displacement micropipettes are frequently used for this…

Uncovering Dimensional Variability in Standard Microtiter Plate Types

By Bradshaw, J., Rodrigues, G., Sawyer, G., Knaide, T., Rogers, A., Sargen, C.
Introduction First created in the 1950’s, microplates have become so common place that in 2004, the Society for Biomolecular Screening (SBS) established standards defining multiple dimensions…

The Use of A Dual Dye Photometric Calibration Method To Identify Possible Sample Dilution From An Automated Multichannel Liquid-Handling System

By Huijin Dong, Zheng Ouyang, Jane Liu, and Mohammed Jemal
A significant difference between the reported volumes delivered by a fluid-filled automated liquid-handling system with eight stainless steel fixed tips was observed when a gravimetric…

Verifying Liquid Handler Performance for Complex or Non-Aqueous Reagents: A New Approach

By Keith J. Albert, John Thomas Bradshaw, Tanya R. Knaide, and Alexis L. Rogers
Multichannel volume dispensing devices, such as automated liquid handlers, are widely used in drug discovery assays and other high-throughput screening processes. The performance of these…

Importance of Integrating a Volume Verification Method for Liquid Handlers: Applications in Learning Performance Behavior

By Keith J. Albert, John Thomas Bradshaw
Nearly all assays performed within a microtiter plate are volume dependent. In turn, all concentrations of biological and chemical components in these assays, as well…

Determining Dilution Accuracy in Microtiter Plate Assays Using Quantitative Dual-Wavelength Absorbance Method

By John Thomas Bradshaw, Richard H. Curtis, Tanya R. Knaide, and Benjamin W. Spaulding
Dilution-based volume transfer steps are commonly performed for many types of microtiter plate-based assays. Because the exact concentrations of target samples in solution are typically…