Speed up assay automation and ensure performance with streamlined liquid class optimization
Getting liquid class settings right is a critical, but often neglected aspect of automating an assay that can reduce CVs and improve reproducibility.
Are sub-optimal liquid class settings holding your assay back?
One of the biggest challenges when automating an assay is getting the liquid classes right. Without optimization, both the accuracy and precision of liquid transfers can be negatively impacted, leading to increased variability in assay results. Yet the importance of liquid class optimization is often underestimated, with many labs relying on their automated liquid handler’s built-in liquid classes. The problem with this approach is that liquid classes are unique to the liquid being transferred, the tips and plates used in the transfer, the temperature of the liquid, and the specific liquid handler being used. With so many variables, labs that want the best assay performance really need to spend more time optimizing liquid classes. The good news is that by using an MVS (Multichannel Verification System), spending “more” time optimizing liquid classes doesn’t have to mean wasting a lot of time.
Nathaniel Hentz, PHD
Director, Scientific Market Development
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If you would like more details on how Artel can support your assay, whether it is troubleshooting, optimization, or assay transfer, don’t hesitate to reach out—we are here to help.
Liquid classes explained
Challenging liquid transfers, such as ones involving 6 or more solutions with low surface tension, are common in molecular biology assays and experienced manual pipette operators often adjust their technique without thinking. They do things like slowing down the pipetting speed, pausing more (or less), or adding in air gaps. But when an assay is transferred to an automated system, those adjustments need to be explicitly programmed into the liquid handler to ensure accurate and precise liquid handling. These adjustments are implemented by changing the liquid class settings.
MVS Multichannel Verification System
Do you know if your automated liquid handler volumes are on target, run after run?
Verify volume transfer precision and accuracy in your lab and simplify regulatory compliance with the easy-to-use MVS.
The MVS streamlines liquid class optimization
Optimizing liquid classes can seem tedious but with an MVS, the process is fast and straightforward. Simply set up your assay and use the MVS reagent that best mimics your liquid. You can test different liquid class settings and then use the MVS to measure how the setting affects liquid handling accuracy and precision.
The above statistics presented in the “Before and After” ALH liquid class optimization figure demonstrate how optimizing liquid classes can improve accuracy and precision, ensuring reproducible results.
Optimizing liquid classes gets assays into production faster
Working with well-defined liquid classes can cut the time it takes to get an assay into production and improve assay quality by reducing variability stemming from liquid handling. With an MVS, getting this important step done is transformed from challenge into challenge-solved.
Are you ready to take your assay optimization to the next level with an MVS? Contact us to get started.
Easily ensure accurate and precise volume transfers
And, of course, the MVS is a great tool to ensure that your liquid handlers are performing as expected by making it easy to perform weekly, monthly, quarterly, semi-annual, or annual volume verification checks as needed for your regulated or non-regulated lab.
Get extra support with Artel Services, Training and Consulting Support
Need expert help with any of these tasks? Artel offers a complete suite of consulting services including automated liquid handler calibration, assay optimization support, proper pipette technique assessment, industry and ISO guidance, training, and more.